Large-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation.
نویسندگان
چکیده
This report describes a 200- to 300-fold increase in the quantity of ribonucleic acud tumor virus particles previously isolated at one time by zonal centrifugation.
منابع مشابه
Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
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عنوان ژورنال:
- Applied microbiology
دوره 26 3 شماره
صفحات -
تاریخ انتشار 1973